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Highlights Novel laser-induced transfer method for fabricating glucose sensors from recycled e-waste copper, offering a sustainable and cost-effective solution. Unique heterogeneous Cu x O nano-skeletons derived from discarded printed circuit boards exhibiting exceptional glucose-sensing performance (sensitivity: 9.893 mA mM−1 cm−2, detection limit: 0.34 μM). Miniaturized glucose detection device, optimized for scalability and portability, revolutionizing diabetes management and patient care.