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The content of bounded amino acids in six entomopathogenic fungi was identified and determined. Analyzing the elements characterizing the pathogenicity of individual species of fungi based on infectivity criteria, ranges of infected hosts, and the ability to induce epizootics, these can be ranked in the following order: <i>Isaria farinosa, Isaria tenuipes, Isaria fumosorose, Lecanicillium lecanii, Conidiobolus coronatus, Isaria coleopterorum.</i> These fungi represent two types of <i>Hyphomycetales-Paecilomyces</i> Bainier and <i>Verticillium Nees</i> ex Fr. and one type of <i>Entomophtorales-Conidiobolus</i> Brefeld. Our study indicates that there are significant quantitative and qualitative differences of bounded amino acids in the entomopathogenic fungal strains contained in the mycelium between high and low pathogenicity strains. The richest composition of bounded amino acids has been shown in the mycelium of the <i>Isaria farinosa</i> strain, which is one of the most commonly presented pathogenic fungi in this group with a very wide range of infected hosts and is the most frequently recorded in nature as an important factor limiting the population of insects.