Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach
oleh: Arivusudar Marimuthu, Tai-Chung Huang, Lakshmi Dhevi N. Selvan, Santosh Renuse, Raja Sekhar Nirujogi, Praveen Kumar, Sneha M. Pinto, Sudha Rajagopalan, Akhilesh Pandey, H.C. Harsha, Aditi Chatterjee
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2014-09-01 |
Deskripsi
miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.