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Background: Thyroid disorders have become much more common in the present days and so are its resultant complications or sequelae. There are marked variations in thyroid function with age and sex, evident in all of the in vitro hormone and protein measurements. Objective: To correlate the thyroid status with age and gender. Methods: 500 subjects of all age group and both genders from the general population of Bagalkot, Karnataka were included in the study. Subjects suffering from thyroid disorders were excluded. Non-Fasting venous samples were collected from all the subjects, serum T3, T4, and TSH levels were estimated by microplate immunoenzymometric assay. Statistical analysis was done by ANOVA and unpaired ‘t’ test. Results: It was observed that T3 and T4 levels declined but TSH levels raised, as the age advanced. T3 and T4 levels were lower and TSH levels higher in female subjects as compared to male subjects, in the age group of 21 – 40 years. Conclusion: The variability in the thyroid status has to be borne in mind during evaluation and treatment of thyroid disorders.